Normal view MARC view ISBD view

Practical Biochemistry: Principles and Techniques (Record no. 12439)

MARC details
000 -LEADER
fixed length control field	04993nam a2200217 a 4500
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
ISBN	9780521799652
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
ISBN	0521799651
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number	574.192
Item number	PRA
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name	Wilson, Keith
245 ## - TITLE STATEMENT
Title	Practical Biochemistry: Principles and Techniques
250 ## - EDITION STATEMENT
Edition statement	5th edition
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication	Cambridge :
Name of publisher	Cambridge University Press,
Year of publication	©2000
300 ## - PHYSICAL DESCRIPTION
Number of Pages	xviii, 784 pages :
Other physical details	illustrations ; 26 cm.
490 ## - SERIES STATEMENT
Series statement	Cambridge low price editions.
500 ## - GENERAL NOTE
General note	Included Index
505 ## - FORMATTED CONTENTS NOTE
Formatted contents note	General principles of biochemical investigations. Nature of biochemistry. Bioenergetics. Methods for investigating metabolism. Practical considerations. In vivo models. In vitro models. Microscopy. Molecular biology and basic techniques. Components and primary structure of nucleic acids. Genes and genome complexity. Nature of the genetic code. Cellular location of nucleic acids. Cellular functions of DNA. Manipulation of nucleic acids: basic tools and techniques. Isolation and separation of nucleic acids. Restriction mapping of DNA fragments. Nucleic acid blotting methods. Gene probe derivation. Labelling DNA gene probe molecules. The polymerase chain reaction. Nucleotide sequencing of DNA. Bioinformatics and the Internet. Molecular cloning and gene analysis. Constructing gene libraries. Cloning vectors. Hybridisation and gene probes. Screening gene libraries. Applications of gene cloning. Expression of foreign genes. Analysing genes and gene expression. Analysing whole genomes. Molecular biotechnology and applications. Immunochemical techniques. Production of antibodies. Purification and fragmentation of immunoglobulins. Immunoprecipitation. Labelling antibodies. Immunoblotting. Immunoassays. Immunohisto/ cytochemistry. Affinity and avidity. Immunochemical use of surface plasmon resonance. Centrifugation techniques. Basic principles of sedimentation. Centrifuges and their uses. Design and care of preparative rotors. Sample containers. Separation methods in Preparative ultracentrifuges. Performing density gradient separations. Selection, efficiency and applications of preparative rotors. Analysis of subcellular fractions. Some applications of the analytical ultracentrifuge. Safety aspects in the use of centrifuges. Protein structure, purification and characterisation. Ionic properties of amino acids and proteins. Protein structure. Protein purification. Protein structure determination. Biomolecular interactions: Enzymes. Receptor-ligand binding. Enzymes: characteristics and nomenclature. Enzyme steady-state kinetics. Enzyme assays. Substrate assays. Enzyme pre-steady-state kinetics. Enzyme active sites and catalytic mechanisms. Immobilised enzymes. Cellular control of metabolic activity. Biomolecular interactions: Cell surface receptors and transporters. Cell surface receptor classification. Quantitative aspects pf receptor-ligand binding. Receptor structures. Mechanisms of signal transduction. Signal amplification. Membrane transport process. Physical diffusion. Facilitated transport. Active transport and ion channels. Receptor-mediated endocytosis. Spectroscopic techniques: Atomic and molecular electronic spectroscopy. Y-ray spectroscopy and Y-ray resonance spectroscopy. X-ray spectroscopy. Ultraviolet and visible light spectroscopy. Spectrofluorimetry. Circular dichroism spectroscopy. Turbidimetry and nephelometry. Luminometry. Atomic spectroscopy. Lasers. Spectroscopic techniques: Vibrational spectroscopy and electron and nuclear spin orientation in magnetic fields. Infrared and Raman spectroscopy. Electron spin resonance spectroscopy. Nuclear magnetic resonance spectroscopy. Mass spectrometric techniques. Mass spectrometer. Electron impact ionisation. Chemical ionisation. Field ionisation. Ion desorption methods. Ion evaporation methods. Analysers. Detectors. Tandem mass spectrometry. Electrophoretic techniques. Support media. Electrophoresis of proteins. Electrophoresis of nucleic acids. Capillary electrophoresis. Chromatographic techniques. Chromatography theory and practice. Low pressure column chromatography. Adsorption chromatography. Partition chromatography. Ion-exchange chromatography. Molecular exclusion (permeation) chromatography. Affinity chromatography. Gas-liquid chromatography. Thin-layer (planar) chromatography. Selection of a chromatographic system. Radioisotope techniques. Nature of radioactivity. Detection and measurement of radioactivity. Other practical aspects of counting radioactivity and analysis of data. Inherent advantages and restrictions of radiotracer experiments. Safety aspects. Applications of radioisotopes in the biological sciences. Electrochemical techniques. Principles of electrochemical techniques. Redox reactions. The pH electrode. Ion-selective and gas-sensing electrodes. The Clark oxygen electrode. Electrochemical detectors for HPLC. Biosensors
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term	Biochemistry -- Methodology.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term	Biochemie -- Metodeleer.
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name	Walker, John
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type	Reference Books

Holdings
Collection code	Home library	Current library	Shelving location	Date acquired	Source of acquisition	Cost, normal purchase price	Full call number	Accession Number	Koha item type
Reference	Main Library	Main Library	Reference	02/08/2005	Purchased	360.00	574.192 PRA	007115	Reference Books